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KMID : 0357319940290010109
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Journal of the Korean Society for Microbiology
1994 Volume.29 No. 1 p.109 ~ p.116
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Detection and Characterization of Hepatitis C virus in Korean non-A, non-B Hepatitis Patients
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주용규
서동진/김연수/백락주/김금룡/이호왕
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Abstract
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To characterize hepatitis C virus stains present in Korean non-A, non-B hepatitis, RNA was extracted from patients' sera using guanidium thiocyanate-phenol-chloroform mixture. RNA was then examined with a combined assay of reverse transcription
and
polymerase chain reaction, using 4 different primer pairs derived from conserved regions in 5' -NCR, and putative core, E2/NS1, NS3 region of the isolates from the United States, Japan and France.
Among 147 cases, hepatitis C virus RNA could be detected from 106(72.1%) cases using 5' -NCR primer; whereas positive rates againt core, E2/NS1. NS3 primers were 46(31.3%), 27(18.4%), 61(41.5%), respectively.
We can divide these 106 cases into 6 patterns based upon reactivity against each 4 primer pairs Among these 106 cases, 29(27.4%) show reactivity against 5' -NCR primer only. These findings indicate that there are considerable heterogeneity in
hepatitis
C virus genomes in Korea.
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